Microbubbles (MBs) have been employed in numerous studies to facilitate focused ultrasound actions (FUS). Their use as FUS enhancer is particularly envisaged for intracranial procedures in order to overcome the obstacle posed by the presence of the skull, which in certain case hamper treatments such as such as blood brain barrier (BBB) opening or thermal ablation. While the influence of different FUS and MBs parameters have been largely studied, less effort has been focused on MBs properties and their development for FUS; also little is known about the behaviour of MBs in the normal brain as well as in different pathologies such as brain tumors. Recent studies showed that MBs distribution is dependent on the brain area under study, as well as on the way of administration. Therefore a more thoroughly understanding of different MBs interaction with FUS, as well as how they behave according to their distribution in different brain areas and administration, is highly desirable. The aim of this study is a proof-of-concept study of the potential variability of ultrasound-induced activation of MBs for BBB opening and MBs enhanced ablation that may be caused by the spatial heterogeinity of MBs distribution in the brain and their differences in MBs composition and size.