Focused ultrasound (FUS) has recently been demonstrated to have the efficacy and
safety for selective targeting of deep-seated regions of the brain (Elias et al., 2016).
The objective of the proposed research program is to utilize FUS to selectively open
the blood brain barrier (BBB) at the site of stroke-induced injury. We will then
systemically deliver a prospectively isolated and purified population of mouse neural
stem cells (NSC) tagged with a fluorescent marker and study the rate of integration of
this population of cells into the site of selective BBB opening and in the rest of the
brain. A control group will be used to assess the efficacy of FUS-induced BBB opening.
If FUS can be safely used to selectively open the BBB for systemically administered
cell transplantation into the CNS, it would provide a minimally invasive modality for
treatment and regeneration in the post-stroke period.