Microbubbles (MBs) are currently used in different clinical settings, especially for imaging with the contrast-enhanced ultrasound (CEUS) technique. Recently, MBs have been used during neurosurgical procedures to highlight different pathologies. MBs therapeutic usage is envisaged in combination with focused ultrasound (FUS) to perform treatments such as blood brain barrier
(BBB) opening and MBs enhanced ablation. However, distribution and localization of MBs in different areas of the normal human brain and in pathological conditions, and their concentration over time are not yet fully known. FUS treatments are therefore limited because of this lack of data.