The annual cancer incidence, tumor type and sites in canine patients are comparable to human cancer. Specifically, canine oral squamous and non-squamous masses are often well-established when detected in the oral cavity, and metastasize aggressively. In preliminary studies, we achieved promising local remission rates of canine oral cancers with focused ultrasound (FUS). Additionally, we found that FUS treatment removed the tumor-mediated immunosuppression in murine melanoma by expanding CD4+ and CD8+ T cells, and polarized macrophages to the tumor-suppressing M1 phenotype. Building on our promising preliminary data, in this project, we will perform a pilot dog clinical trial to determine "time to metastasis" as an initial index of the systemic antitumor immune effect of FUS by mmunopathological and radiological means, informing feasibility for human trials.